Male factor infertility is a common reason couples seek Cyprus IVF treatment. When sperm is not found in the ejaculate (a condition called azoospermia) or when sperm count is extremely low, doctors may recommend surgical sperm retrieval techniques such as TESA and TESE.
If you are searching for TESA in Cyprus, TESE Cyprus, sperm retrieval for IVF in North Cyprus, or male infertility treatment in Cyprus, this guide explains what these procedures are, who needs them, how the process works, and how sperm retrieved through TESA/TESE is used in IVF–ICSI.
TESA is a minimally invasive procedure where sperm is aspirated (withdrawn) from the testicle using a fine needle. It is typically performed under local anesthesia or mild sedation.
Main idea:
A needle is inserted into the testis and a small sample is aspirated. The lab then searches the sample for sperm cells that can be used for ICSI (intracytoplasmic sperm injection).
TESE involves taking a small piece of testicular tissue through a minor surgical incision. The tissue is examined in the laboratory to find sperm.
Main idea:
A small sample of testicular tissue is removed, and sperm cells are extracted from within that tissue.
TESA/TESE are usually considered when sperm cannot be collected naturally from ejaculation or when the sperm count is too low for standard IVF.
Common reasons include:
Azoospermia (no sperm in semen)
Obstructive azoospermia (OA): sperm production may be normal, but a blockage prevents sperm from entering the ejaculate.
Non-obstructive azoospermia (NOA): sperm production is reduced; retrieval can still be possible in some cases.
Severe male factor infertility
Failed sperm retrieval from ejaculation on the day of IVF
Situations where sperm must be collected directly due to medical or anatomical reasons
This is why many patients researching North Cyprus IVF also search for sperm retrieval Cyprus and azoospermia treatment Cyprus.
TESA: needle aspiration (less invasive)
TESE: small surgical extraction (more tissue available for sperm search)
TESA: commonly for obstructive azoospermia or when sperm production is likely
TESE: when TESA is unlikely to retrieve sperm or when a deeper tissue search is needed
TESE may provide more tissue for lab processing, which can increase chances in certain scenarios, especially in difficult male factor cases.
Your Cyprus IVF team chooses the method based on semen analysis results, hormone profile (FSH, LH, testosterone), testicular exam/ultrasound findings, and medical history.
Men diagnosed with azoospermia
Men with a history of vasectomy or suspected blockage
Men with failed ejaculation or severe ejaculatory dysfunction
Men with extremely low sperm counts where freezing is needed
Couples preparing for ICSI in Cyprus where sperm must be retrieved surgically
Before sperm retrieval, the clinic typically evaluates:
Semen analysis (often repeated for confirmation)
Hormone blood tests (FSH, LH, testosterone, prolactin)
Genetic screening where indicated (e.g., Y-chromosome microdeletions, karyotype)
Scrotal ultrasound and physical exam
Review of past surgeries or infections
This stage helps determine whether sperm retrieval is likely and which technique is most appropriate.
In many cases, TESA/TESE is timed with the female partner’s egg retrieval so retrieved sperm can be used immediately for ICSI.
Alternatively, sperm can be:
Retrieved and frozen (cryopreserved) before the IVF cycle
Stored as a backup plan, especially when sperm retrieval may be challenging
This is especially helpful for international patients planning Cyprus IVF travel efficiently.
TESA: usually quick, often under local anesthesia or mild sedation
TESE: minor surgical procedure with a small incision, typically under local anesthesia with sedation or short anesthesia depending on protocol
Most patients go home the same day.
Immediately after retrieval:
The embryology/andrology lab processes the sample
The lab searches for viable sperm cells
If sperm is found, it is prepared for ICSI or frozen for later use
Because retrieved sperm counts are often low and sperm motility may be limited, ICSI is usually the preferred fertilization method.
If enough sperm is retrieved, the clinic may freeze:
extra sperm cells for future IVF cycles
backup material to avoid repeat surgery
retrieved testicular tissue (in some cases, depending on the lab strategy)
Patients searching TESE Cyprus often specifically ask about sperm freezing as part of the plan.
Sperm obtained through TESA or TESE is typically used for:
In ICSI:
a single sperm cell is injected directly into a mature egg
this bypasses the need for high sperm motility and count
it is the standard fertilization method for surgically retrieved sperm
So, many treatment plans in Cyprus IVF combine:
TESA/TESE + ICSI + embryo transfer
Mild discomfort is common
Light swelling or bruising may occur
Most patients return to normal activity quickly
Slightly more recovery time than TESA
Mild pain or tenderness is common
The clinic may recommend avoiding heavy lifting, intense exercise, and sexual activity for a short period
Your clinic will provide individualized post-procedure guidance.
Like any medical procedure, sperm retrieval has possible risks, including:
Mild bleeding, bruising, or swelling
Infection (rare with proper sterile technique)
Temporary discomfort
In some cases, no sperm is found, especially in severe non-obstructive azoospermia
Important note:
TESA/TESE can increase the chance of retrieving sperm, but it does not guarantee that sperm will be found or that pregnancy will occur. Success depends on many factors, including egg quality, embryo development, and uterine receptivity.
Most patients report mild discomfort. Anesthesia or sedation is used depending on the procedure and clinic protocol.
Yes. Many patients choose retrieval and freezing in advance to reduce uncertainty and improve IVF planning.
Often yes. Freezing retrieved sperm can help avoid repeat procedures in future cycles.
Not always. In obstructive azoospermia, retrieval rates are often higher. In non-obstructive azoospermia, success varies depending on the cause and testicular sperm production.
TESA and TESE are important sperm retrieval techniques for couples facing severe male factor infertility or azoospermia. In a well-organized Cyprus IVF treatment plan, these procedures are commonly combined with ICSI, offering many patients a realistic chance of biological parenthood even when no sperm is present in semen.
If you are considering TESA in Cyprus or TESE Cyprus, the safest approach is a complete male fertility evaluation and a personalized plan that aligns sperm retrieval with embryo creation and transfer timing.